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All biomarkers
Hormones

Free testosterone (calculated)

Measured in pg/mL · LOINC 2986-8

Reference ranges

Optimal
1025 pg/mL
functional-medicine target
Standard
521 pg/mL
lab reference range

Optimal ranges are tighter than standard lab ranges and reflect functional-medicine targets, not the populations they were derived from. Reference bands depend on sex, age, and circumstance — Juno's lab analysis layers your profile context onto the printed range when you paste a value.

Peptide relevance

The biologically active fraction of testosterone — the part not bound to SHBG or albumin. Most clinically reliable when CALCULATED from total testosterone + SHBG + albumin (Vermeulen equation) rather than measured by direct immunoassay (the latter is notoriously inaccurate). Equilibrium dialysis is the gold standard but rarely ordered. Symptoms of low T correlate better with free T than total T, especially when SHBG is at the extremes. Same peptide interactions as total testosterone — exogenous androgens (TRT, AAS) raise it; CJC-1295 + Ipamorelin don't directly affect HPG axis but improving body composition can modestly lift free T via lower SHBG. RANGES SHOWN ARE FOR ADULT MEN; women typically <2 pg/mL.

Peptides that influence this marker

Documented to affect this marker. Click through for the full evidence-tiered profile.

Have a recent Free testosterone (calculated) value?

Paste it on the lab analysis page. Juno reads your active stack + (if shared) your medications and conditions, and surfaces plausible contributors with citations. Doesn't diagnose.

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